Course Content:
- Biochemical calculations
- Data mining using GENBANK
- Blasting sequences
- Preparation of insert DNA
- Preparation of vector DNA
- Purification of vector and insert DNA from agarose gel
- Choice of vector and host systems
- Dephosphorylation
- Ligation
- Preparation of competent cells
- Bacterial Transformation
- Screening of clones using
- retardation check
- restriction digestion
- Southern Hybridization
- Varients of PCR
- Amplification of a gene
- RT- PCR
- RAPD
- Laboratory instruments, principle and use