Diploma in Recombinant DNA Technology

Course Content:

Duration: 1 Month
This covers

  1. Microbial techniques,
  2. Cloning and expression of gene and bioinformatics
  3. Polymerase chain reaction and Hybridization Techniques
    • Good laboratory practices
    • Laboratory instruments, principle and use
    • Biochemical calculations
  1. Microbiology Techniques
    1. Aseptic techniques
    2. Preparation of media, plating, streaking, inoculation etc
    3. Phage titration
  2. Cloning and expression of gene and Bioinformatics
    1. Restriction digestion
    2. Agarose gel electrophoresis
    3. Purification of DNA fragment from agarose gel
    4. Genomic DNA isolation from blood
    5. Genomic DNA isolation from bacteria
    6. Genomic DNA isolation from plant
    7. Plasmid isolation
    8. DNA quantitation
    9. Data mining using GENBANK
    10. Blasting sequences
    11. Preparation of vector DNA
    12. Preparation of insert DNA
    13. Purification of vector and insert DNA from agarose gel
    14. Choice of vector and host systems
    15. Dephosphorylation
    16. Ligation
    17. Preparation of competent cells
    18. Transformation
    19. Screening of clones using
      1. retardation check
      2. restriction digestion
  3. Polymerase chain reaction and Hybridization Techniques
    1. Primer design
    2. Amplification of gene
    3. RAPD fingerprinting
    4. RT PCR
    5. PCR-RFLP
    6. Southern Hybridizatio