Module 4: Protein Purification Techniques
Course Content:
Course Content:
- Biochemical calculations
- Buffer preparation
- Precipitation techniques
- Column Chromatography
- Purification of enzyme using ion exchange chromatography
- Gel filtration chromatography for separation of biomolecules
- Purification of enzyme using affinity chromatography
- Protein concentration techniques
- Dialysis
- Enzyme unit determination
- Determination of specific activity
- Protein stabilization
- Purity checks
- SDS-PAGE
- Western Blotting
