Protein purification Techniques

Course Content:

  1. Biochemical calculations
  2. Laboratory instruments, principle and use
  3. Buffer preparation
  4. Precipitation techniques
  5. Purification of enzyme using ion exchange chromatography
  6. Gel filteration chromatography for separation of biomolecules
  7. purification of restriction enzyme using affinity chromatography
  8. Protein concentration techniques
  9. Dialysis
  10. Enzyme unit determination
  11. Determination of Specific activity
  12. Protein stabilization
  13. Purity checks
  14. SDS-PAGE
  15. Western Blotting