Tools in Genetic engineering and Microbiology

Course Content:

  • Good laboratory practices
  • Biochemical calculations
  • Aseptic techniques
  • Preparation of media, plating, streaking, inoculation etc.
  • Phage titration
  • Laboratory instruments, principle and use
  • Restriction digestion
  • Agarose gel electrophoresis
  • Plasmid isolation
  • Ligation
  • Transformation
  • Cloning
  • Polymerase chain reaction